uvb narrowband lamps pl-s 9 w  (Philips Healthcare)

Journal: Journal of Cosmetic Dermatology

Article Title: Synergistic Anti‐Aging Effects of Adipose‐Derived Stem Cell Extracellular Vesicles Loaded With Natural Compounds

doi: 10.1111/jocd.70021

Figure Lengend Snippet: Screening for the highest combined anti‐oxidant activity. (A) Experimental design of the screen for cytotoxicity and anti‐oxidant effect using human keratinocyte HaCaT cells. ROS measurement was performed in HaCaT cells irradiated with UVB and treated with different concentrations of (B) human ASC‐sEVs, (C) selected natural compounds, and (D) human ASC‐sEVs loaded with compounds. * p < 0.05, student's t ‐test. NR, nicotinamide riboside; RES, resveratrol; ROS, reactive oxygen species; UVB, ultraviolet B; VITC, vitamin C.

Article Snippet: The cells were then incubated with different sEV preparations or compounds for 48 h. For ROS measurement, cells were first stained with 25 μM DCFDA (ab113851, Abcam) for 45 min in the dark [ ], then washed with PBS and exposed under UVB Narrowband lamps (PL‐S 9 W, Philips) at 30 mJ/cm 2 .

Techniques: Activity Assay, Irradiation

Journal: Journal of Cosmetic Dermatology

Article Title: Synergistic Anti‐Aging Effects of Adipose‐Derived Stem Cell Extracellular Vesicles Loaded With Natural Compounds

doi: 10.1111/jocd.70021

Figure Lengend Snippet: Screening for the highest combined anti‐wrinkle activity. (A) Experimental design of the screen for cytotoxicity and anti‐wrinkle effect using human keratinocyte HaCaT cells. (B) The morphology of HaCaT cells with or without UVB irradiation. Gene expressions of MMP1 , MMP3 , and PLOD1 were measured in HaCaT cells irradiated with UVB and treated with different concentrations of (C) human ASC‐sEVs, (D) selected natural compounds, and (E) human ASC‐sEVs loaded with compounds. * p < 0.05, student's t ‐test. MMP, matrix metalloproteinase; PLOD1, procollagen‐lysine 2‐oxoglutarate 5‐dioxygenase 1; RES, resveratrol; RET, retinol; UVB, ultraviolet B; VITC, vitamin C.

Article Snippet: The cells were then incubated with different sEV preparations or compounds for 48 h. For ROS measurement, cells were first stained with 25 μM DCFDA (ab113851, Abcam) for 45 min in the dark [ ], then washed with PBS and exposed under UVB Narrowband lamps (PL‐S 9 W, Philips) at 30 mJ/cm 2 .

Techniques: Activity Assay, Irradiation

Journal: Journal of Cosmetic Dermatology

Article Title: Synergistic Anti‐Aging Effects of Adipose‐Derived Stem Cell Extracellular Vesicles Loaded With Natural Compounds

doi: 10.1111/jocd.70021

Figure Lengend Snippet: Screening for the highest combined anti‐melanogenic activity. (A) Experimental design of the screen for cytotoxicity and anti‐melanogenic effect using human melanocyte B16F10 cells. Intracellular and extracellular melanin levels were measured from B16F10 cell culture treated with different concentrations of (B) human ASC‐sEVs, (C) selected natural compounds, and (D) human ASC‐sEVs loaded with compounds. * p < 0.05, student's t ‐test. ARB, arbutin; NR, nicotinamide riboside; RES, resveratrol; RET, retinol; UVB, ultraviolet B; VITC, vitamin C.

Article Snippet: The cells were then incubated with different sEV preparations or compounds for 48 h. For ROS measurement, cells were first stained with 25 μM DCFDA (ab113851, Abcam) for 45 min in the dark [ ], then washed with PBS and exposed under UVB Narrowband lamps (PL‐S 9 W, Philips) at 30 mJ/cm 2 .

Techniques: Activity Assay, Cell Culture